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8. | | PUTI, F. da C.; BECARO, A. A.; PILON, L.; CORREA, D. S.; FERREIRA, M. D. Aplicação de filmes nanoparticulados na conservação de morangos. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 7.; ESCOLA DE NANOTECNOLOGIA, 3., 2013, São Carlos, SP. Anais... São Carlos, SP: Embrapa Instrumentação, 2013. p. 112-113 Editores: Maria Alice Martins, Odílio Benedito Garrido de Assis, Caue Ribeiro, Luiz Henrique Capparelli Mattoso. CD-ROM. Editores: Maria Alice Martins, Odílio Benedito Garrido de Assis, Caue Ribeiro, Luiz Henrique Capparelli Mattoso. Biblioteca(s): Embrapa Instrumentação. |
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12. | | PILON, L. E.; ARCARO, J. R. P.; ZAFALON, L. F.; SANTANA, R. C. M. Identificação de staphylococcus coagulase negativo isolados de glândulas mamárias de novilhas tratadas no pré-parto. Identification of coagulase-negative staphylococci isolated from mammary glands of heifers treated pre-calving In: SIMPÓSIO INTERNACIONAL DE MEDICINA VETERINÁRIA PREVENTIVA, 2013, Jaboticabal. Novas tecnologias, novos desafios- anais. Ars Veterinária, v. 29, supl.1, 2013. Biblioteca(s): Embrapa Pecuária Sudeste. |
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13. | | PILON, L. E.; ARCARO, J. R. P.; ZAFALON, L. F.; SANTANA, R. C. M. Identification of coagulase-negative staphylococci isolated from mammary glands of heifers treated pre-calving. In: SIMPÓSIO INTERNACIONAL DE MEDICINA VETERINÁRIA PREVENTIVA, 2013, Jaboticabal. Novas tecnologias, novos desafios- anais. Ars Veterinária, v. 29, supl.1, 2013. Biblioteca(s): Embrapa Pecuária Sudeste. |
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14. | | PILON, L.; SPRICIGO, P. C.; MOURA, M. R.; MATTOSO, L. H. C.; FERREIRA, M. D. Evaluacion del pardeamiento enzimático de manzanas minimamente procesadas recubiertas com nanopartículas de quitosano. In: CONGRESO IBEROAMERICANO DE TECNOLOGIA POSTCOSECHA Y AGROEXPORTACIONES, 7., 2012, La Plata, Argentina. Resumos...La Plata, Argentina: AITEP, 2012. 1 CD-ROM. não paginado. Biblioteca(s): Embrapa Instrumentação. |
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15. | | PILON, L.; SPRICIGO, P. C.; MOURA, M. R.; MATTOSO, L. H. C.; FERREIRA, M. D. Efeitos de revestimento à base de nanopartículas de quitosana na atividade enzimática de maçãs minimamente processadas. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 7.; ESCOLA DE NANOTECNOLOGIA, 3., 2013, São Carlos, SP. Anais... São Carlos, SP: Embrapa Instrumentação, 2013. p. 94-96 Editores: Maria Alice Martins, Odílio Benedito Garrido de Assis, Caue Ribeiro, Luiz Henrique Capparelli Mattoso. CD-ROM. Editores: Maria Alice Martins, Odílio Benedito Garrido de Assis, Caue Ribeiro, Luiz Henrique Capparelli Mattoso. Biblioteca(s): Embrapa Instrumentação. |
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Registros recuperados : 96 | |
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Registro Completo
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
03/08/2020 |
Data da última atualização: |
03/08/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
BRAIR, V. L.; MAIA, A. L. R. S.; CORREIA, L. F. L.; BARBOSA, N. O.; SANTOS, J. D. R.; BRANDÃO, F. Z.; FONSECA, J. F. da; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G. |
Afiliação: |
VIVIANE L. BRAIR, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; ANA LUCIA R. S. MAIA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; LUCAS FRANCISCO L. CORREIA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; NATHALIA O. BARBOSA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; JULIANA D. R. SANTOS, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; FELIPE Z. BRANDÃO, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; JEFERSON FERREIRA DA FONSECA, CNPC; RIBRIO IVAN T. P. BATISTA, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; JOANNA M. G. SOUZA-FABJAN, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil. |
Título: |
Gene expression patterns of in vivo-derived sheep blastocysts is more affected by vitrification than slow freezing technique. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Cryobiology, v. 95, p. 110-115, 2020. |
DOI: |
https://doi.org/10.1016/j.cryobiol.2020.05.009 |
Idioma: |
Inglês |
Conteúdo: |
Transfer of fresh sheep embryos frequently results in higher pregnancy rate compared to cryopreserved ones, possibly due to a failure in the communication between the cryopreserved embryo and the endometrium during pre-implantation and pregnancy establishment. Thus, this study assessed the effect of sheep embryo cryopres-ervation (slow freezing or vitrification) on embryo survival rate and expression of genes related to trophectoderm differentiation (CDX2), pluripotency maintenance (NANOG), cell proliferation (TGFB1), mitochondrial activity (NRF1) and apoptosis (BAX and BCL2). Superovulation (n ¼32 ewes) was performed and embryos were transcervically collected. One hundred good quality (Grade I and II) embryos were allocated into three groups: fresh embryos (CTL; n ¼15), slow freezing (SF; n ¼42) or vitrification (VT; n ¼43). After thawing/warming, three pools of five blastocysts per group were used for RT-qPCR; the remaining 55 embryos were cultured in vitro in SOFaa medium at 38.5 �C and 5% CO2 (SF: n ¼27 and VT: n ¼28). Survival rate of SF and VT were, respectively, 29.6% (8/27) and 14.2% (4/28) at 24 h; and 48.1% (13/27) and 32.1% (9/28) at 48 h (P >0.05). Only CDX2 was affected (up-regulated, P <0.05) in both groups compared to CTL. The BAX transcript was upregulated in VT, compared to SF group. The VT increased (P <0.05) the expression of all genes, except for NANOG and NRF1, when compared to the CTL. In conclusion, although in vitro survival was similar between techniques, VT led to increased changes in blastocyst gene expression compared to CTL and SF. MenosTransfer of fresh sheep embryos frequently results in higher pregnancy rate compared to cryopreserved ones, possibly due to a failure in the communication between the cryopreserved embryo and the endometrium during pre-implantation and pregnancy establishment. Thus, this study assessed the effect of sheep embryo cryopres-ervation (slow freezing or vitrification) on embryo survival rate and expression of genes related to trophectoderm differentiation (CDX2), pluripotency maintenance (NANOG), cell proliferation (TGFB1), mitochondrial activity (NRF1) and apoptosis (BAX and BCL2). Superovulation (n ¼32 ewes) was performed and embryos were transcervically collected. One hundred good quality (Grade I and II) embryos were allocated into three groups: fresh embryos (CTL; n ¼15), slow freezing (SF; n ¼42) or vitrification (VT; n ¼43). After thawing/warming, three pools of five blastocysts per group were used for RT-qPCR; the remaining 55 embryos were cultured in vitro in SOFaa medium at 38.5 �C and 5% CO2 (SF: n ¼27 and VT: n ¼28). Survival rate of SF and VT were, respectively, 29.6% (8/27) and 14.2% (4/28) at 24 h; and 48.1% (13/27) and 32.1% (9/28) at 48 h (P >0.05). Only CDX2 was affected (up-regulated, P <0.05) in both groups compared to CTL. The BAX transcript was upregulated in VT, compared to SF group. The VT increased (P <0.05) the expression of all genes, except for NANOG and NRF1, when compared to the CTL. In conclusion, although in vitro survival was similar between... Mostrar Tudo |
Palavras-Chave: |
Apoptosis regulator; Embryo cryopreservation; Embryonic metabolism; Reproductive biotechnologies. |
Thesaurus NAL: |
Embryo implantation; Embryo transfer; Ewes. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02602naa a2200313 a 4500 001 2124160 005 2020-08-03 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.cryobiol.2020.05.009$2DOI 100 1 $aBRAIR, V. L. 245 $aGene expression patterns of in vivo-derived sheep blastocysts is more affected by vitrification than slow freezing technique.$h[electronic resource] 260 $c2020 520 $aTransfer of fresh sheep embryos frequently results in higher pregnancy rate compared to cryopreserved ones, possibly due to a failure in the communication between the cryopreserved embryo and the endometrium during pre-implantation and pregnancy establishment. Thus, this study assessed the effect of sheep embryo cryopres-ervation (slow freezing or vitrification) on embryo survival rate and expression of genes related to trophectoderm differentiation (CDX2), pluripotency maintenance (NANOG), cell proliferation (TGFB1), mitochondrial activity (NRF1) and apoptosis (BAX and BCL2). Superovulation (n ¼32 ewes) was performed and embryos were transcervically collected. One hundred good quality (Grade I and II) embryos were allocated into three groups: fresh embryos (CTL; n ¼15), slow freezing (SF; n ¼42) or vitrification (VT; n ¼43). After thawing/warming, three pools of five blastocysts per group were used for RT-qPCR; the remaining 55 embryos were cultured in vitro in SOFaa medium at 38.5 �C and 5% CO2 (SF: n ¼27 and VT: n ¼28). Survival rate of SF and VT were, respectively, 29.6% (8/27) and 14.2% (4/28) at 24 h; and 48.1% (13/27) and 32.1% (9/28) at 48 h (P >0.05). Only CDX2 was affected (up-regulated, P <0.05) in both groups compared to CTL. The BAX transcript was upregulated in VT, compared to SF group. The VT increased (P <0.05) the expression of all genes, except for NANOG and NRF1, when compared to the CTL. In conclusion, although in vitro survival was similar between techniques, VT led to increased changes in blastocyst gene expression compared to CTL and SF. 650 $aEmbryo implantation 650 $aEmbryo transfer 650 $aEwes 653 $aApoptosis regulator 653 $aEmbryo cryopreservation 653 $aEmbryonic metabolism 653 $aReproductive biotechnologies 700 1 $aMAIA, A. L. R. S. 700 1 $aCORREIA, L. F. L. 700 1 $aBARBOSA, N. O. 700 1 $aSANTOS, J. D. R. 700 1 $aBRANDÃO, F. Z. 700 1 $aFONSECA, J. F. da 700 1 $aBATISTA, R. I. T. P. 700 1 $aSOUZA-FABJAN, J. M. G. 773 $tCryobiology$gv. 95, p. 110-115, 2020.
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